Transcranial Functional Ultrasound Imaging in Freely Moving Awake Mice and Anesthetized Young Rats without Contrast Agent
Elodie Tiran, Jérémy Ferrier, Thomas Deffieux, Jean-Luc Gennisson, Sophie Pezet, Zsolt Lenkei, Mickaël Tanter
Ultrasound in Medicine and Biology (2017)
The first proof of concept of transcranial fUS imaging in awake rodents has been published this month (May 2017) in Ultrasound for Medicine and Biology. Transcranial images of the brain vasculature can be obtained in mice until 1 year of age and rats until 1 month of age, without requiring any contrast agent or invasive surgery. Our method was validated by imaging the brain activation as a response to whisker stimulation on awake and freely moving mice.
Cerebral blood volume of young rats between 5 and 45 post-natal days old. Top row: through skull and skin. Bottom row: through skull after skin removal.
For more information: http://dx.doi.org/10.1016/j.ultrasmedbio.2017.03.011