4D microvascular imaging based on ultrafast Doppler tomography.
NeuroImage, November 2015
Our team “Wave physics for medicine” (Inserm Unit 979) led by Mickaël Tanter, in collaboration with the Inserm Unit 1130 “Neuroscience Paris Seine” led by Ivan Cohen, has recently published a new article in the NeuroImage journal.
This paper presents a new imaging technique called ultrafast Doppler tomography (UFD-T) that enables 4D microvascular imaging of brain hemodynamics in rodentsby combining ultrasound ultrafast Doppler and tomographic reconstruction for 3D imaging. The technique reaches 100 μm resolution and is sensitive to very slow blood flow (1 mm/s) with 4D capabilities during one cardiac cycle and opens the way to 4D imaging on awake and moving animals. 4D ultrasound microvascular imaging in vivo could become a valuable tool for the study of brain hemodynamics, such as cerebral flow autoregulation or vascular remodeling after ischemic stroke recovery, and, more generally, tumor vasculature response to therapeutic treatment.
“ UFD-T is a powerful tool both in the field of fUltrasound and preclinical research. Indeed having access to the reconstruction of a large 3D vascular volume (of the order of the several cubic centimeters) with a resolution down to 100µm isotropic is very informative at several levels. The recent publication of the fUSImagine group on combined EEG/fUltrasound in awake and mobile rats (Nature methods) highlights that this kind of portable setup will be the spearhead of this technology. In this context, where 2D fUS can be conducted in an arbitrary plane, being able to acquire on the fly the local vascular geometry in 3D to precisely locate the different cerebral structures and take into account interindividual variations is unvaluable. ” explains Charlie Demene, the first Author of this research work and postdoctoral research fellow at the Langevin Institute – Wave Physics for Medicine.
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